Literature summary for 2.6.1.7 extracted from

Nadvi, N.A.; Salam, N.K.; Park, J.; Akladios, F.N.; Kapoor, V.; Collyer, C.A.; Gorrell, M.D.; Church, W.B.
High resolution crystal structures of human kynurenine aminotransferase-I bound to PLP cofactor, and in complex with aminooxyacetate (2017), Protein Sci., 26, 727-736 .

Search for more literature for 2.6.1.7

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Sf9 insect cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with pyridoxal 5'-phosphate and aminooxyacetate, hanging drop vapor diffusion method, using 200 mM sodium acetate, 29% (w/v) PEG4000 in 100 mM Tris, pH 7.3	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
Aminooxyacetate	-	Homo sapiens
aminooxyphenylpropionic acid	-	Homo sapiens
L-phenylalanine	-	Homo sapiens
L-tryptophan	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q16773	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE Sepharose column chromatography, Blue Sepharose column chromatography, and Superdex 200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-kynurenine + 2-oxobutyrate	-	Homo sapiens	kynurenic acid + 2-aminobutanoate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
KAT-I	-	Homo sapiens
kynurenine aminotransferase-I	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0057	-	at pH 8.0 and 45°C	Homo sapiens	aminooxyphenylpropionic acid
0.0131	-	at pH 8.0 and 45°C	Homo sapiens	Aminooxyacetate
1	-	at pH 8.0 and 45°C	Homo sapiens	L-tryptophan
3	-	at pH 8.0 and 45°C	Homo sapiens	L-phenylalanine

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Release 2023.1 (January 2023)